Cryopreservation and genetic stability of Dendranthema grandiflora Tzvelev in vitro cultures
Martín, Carmen; González-Benito, M. Elena (2009)
Martín, Carmen
González-Benito, M. Elena
Julkaisusarja
Agricultural and Food Science
Volyymi
18
Numero
2
Sivut
129-135
MTT Agrifood Research Finland The Scientific Agricultural Society of Finland
2009
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi-fe2015090311182
http://urn.fi/URN:NBN:fi-fe2015090311182
Tiivistelmä
Shoots apices of different chrysanthemum species and varieties have been cryopreserved following different procedures, which are reviewed and compared in this paper. The results obtained are not only methoddependant but also cultivar-dependant. When several methods were compared with a cultivar best results were generally obtained with vitrification procedures than with encapsulation-dehydration. Not much attention has been given to the stability of the recovered chrysanthemum shoots but there are indications of appearance of somaclonal variants, which in some cases has been explained as being due to the chimeric nature of the cultivar employed. Molecular-based stability studies have also shown the appearance of variants.