Effect of dietary linseed supplements on ù-3 PUFA content and on IGF-1 expression in broiler tissues
Saprõkina, Zinaida; Karus, Avo; Kuusik, Sirje; Tikk, Harald; Järv, Peeter; Soidla, Riina; Lember, Aleksander; Kaldmäe, Helgi; Karus, Virge; Roasto, Mati (2009)
Agricultural and Food Science
MTT Agrifood Research Finland The Scientific Agricultural Society of Finland
Julkaisun pysyvä osoite on
The aim of this study was to evaluate the effect of ù-3 PUFA-rich linseed-supplemented diet on the ù-6/ù-3 PUFA content and IGF-I mRNA expression in broiler tissues. Broilers (50 of 21 d old and 90 of 42 d old) were divided into groups according to dietary additives: 1.5% linseed oil (LO), 3.0% LO, 15% linseed cake (LC) and 30% LC and to the duration of modified feeding (1 21, 1 42 and 22 42 days). PUFA content in breast muscles was assayed by gas chromatography and IGF-I mRNA content in liver, muscle and leukocytes was measured by one step RT-PCR. The experimental diets improved the ù-6/ù-3 PUFA ratio through a threefold increase in the ù-3 PUFA content in the breast muscle. The changes in PUFA content were accompanied by changes in IGF-1 gene expression levels in tissues. Therefore, both paracrine and autocrine manner of IGF-1 action are likely to be involved. 15% LC at starting period or 1.5% LO in final diet can be advised as optimal for enriching broiler meat with ù-3 fatty acids.