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Functional profiling of the endometrium transcriptome during preimplantation development in Finnsheep, Texel and their F1 crosses

dc.contributor.authorPokharel, Kisun
dc.contributor.authorPeippo, Jaana
dc.contributor.authorLi, Meng-Hua
dc.contributor.authorKantanen, Juha
dc.contributor.departmentid4100111010
dc.contributor.departmentid4100210910
dc.contributor.departmentid4100210910
dc.contributor.orcidhttps://orcid.org/0000-0002-4924-946X
dc.contributor.orcidhttps://orcid.org/0000-0002-5841-2898
dc.contributor.orcidhttps://orcid.org/0000-0001-6350-6373
dc.contributor.organizationLuonnonvarakeskus
dc.date.accessioned2020-10-05T07:56:42Z
dc.date.accessioned2025-05-27T18:19:59Z
dc.date.available2020-10-05T07:56:42Z
dc.date.issued2020
dc.description.abstractCarefully coordinated interaction between the endometrium and embryo is critical for the establishment and main-tenance of pregnancy in mammals. By exploring the gene expression dynamics of this tissue during preimplanta-tion development, we may be able to get insight into the genetic mechanisms of reproduction during early preg-nancy. Here, we have performed comparative transcriptome profiling of the endometrium in response to spheri-cal (Day 7 to Day 12) and elongated (Day 13 to Day 17) embryos in Finnsheep, Texel and their F1 crosses using RNA sequencing (RNA-seq) approach. A total of 21125 genes were expressed in our dataset of which 554 were signifi-cantly (absolute log2 fold change > 2.5; adjusted p-value < 0.01) upregulated in the endometrium with elongated embryos. Highly abundant autosomal genes in the endometrium were associated with biological processes such as facilitation of maternal recognition of pregnancy, trophoblast elongation and implantation (LGALS15, CST3, CST6, and EEF1A1). Several endogenous retroviruses (ERVs) including a novel ERV gene located in a reduced FecL locus potentially associated with sheep prolificacy were expressed in our dataset. Comparative transcriptome profiling of the endometrium having spherical and elongated embryos revealed distinct gene expression patterns. Genes that were upregulated in response to elongated embryos indicated the importance of immune system at the maternal-embryo interface prior to implantation.
dc.description.vuosik2020
dc.format.bitstreamtrue
dc.format.pagerange331-345
dc.identifier.olddbid488870
dc.identifier.oldhandle10024/546331
dc.identifier.urihttps://jukuri.luke.fi/handle/11111/5743
dc.identifier.urnURN:NBN:fi-fe2020100578035
dc.language.isoen
dc.okm.corporatecopublicationei
dc.okm.discipline412
dc.okm.internationalcopublicationon
dc.okm.openaccess1 = Open access -julkaisukanavassa ilmestynyt julkaisu
dc.okm.selfarchivedon
dc.publisherScientific Agricultural Society of Finland
dc.relation.doi10.23986/afsci.90523
dc.relation.ispartofseriesAgricultural and food science
dc.relation.issn1459-6067
dc.relation.issn1795-1895
dc.relation.numberinseries4
dc.relation.volume29
dc.rightsCC BY 4.0
dc.source.identifierhttps://jukuri.luke.fi/handle/10024/546331
dc.subject.ysoendogenous retrovirus,
dc.subject.ysoembryo
dc.subject.ysoFinnsheep
dc.subject.ysoprostaglandins
dc.subject.ysoimmune processes
dc.subject.ysotexel
dc.subject.ysotranscriptome
dc.teh41007-00018400
dc.titleFunctional profiling of the endometrium transcriptome during preimplantation development in Finnsheep, Texel and their F1 crosses
dc.typepublication
dc.type.okmfi=A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä|sv=A1 Originalartikel i en vetenskaplig tidskrift|en=A1 Journal article (refereed), original research|
dc.type.versionfi=Publisher's version|sv=Publisher's version|en=Publisher's version|

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