Fungal and beetle diversity in deciduous fine woody debris in spruce-dominated forests in relation to substrate quantity and quality

Abstract

Deciduous fine woody debris (DFWD) is a common deadwood substrate type in boreal conifer-dominated forests, but it is usually present in low volumes, and its importance for deadwood dependent biodiversity is poorly understood. In this study, we investigated how DFWD-associated fungal and beetle diversity depends on local substrate availability and quality, and how species diversity differs between DFWD and coarse deciduous deadwood (birch logs) in boreal mixed spruce-dominated forests in southern Finland. We studied 25 forest plots (each 0.16 ha), measuring and sampling all pieces of DFWD with a diameter of 2–5 cm and minimum length of 50 cm. Wood-inhabiting fungi were surveyed from wood samples by DNA metabarcoding and saproxylic beetles were surveyed by bark sieving. Our results showed a clear positive relationship between DFWD abundance and the diversity of fungi and beetles. Tree species and decay class diversity were not important in explaining fungal and beetle diversity or community composition, possibly due to low degree of variation in DFWD quality among the study plots. DFWD hosted more diverse fungal assemblages than birch logs, including species of conservation concern, while no red-listed beetle species were observed on DFWD. Overall, species assemblages associated with fine and coarse deciduous deadwood were non-nested. Thus, DFWD represents a non-redundant complementary deadwood resource type alongside coarse deciduous deadwood in boreal forests.