The Jan Sjödin faba bean mutant collection: morphological and molecular characterization
BioMed Central
2024
s41065-024-00339-7.pdf - Publisher's version - 1.96 MB
How to cite: Khazaei, H., Carlson-Nilsson, U. & Schulman, A.H. The Jan Sjödin faba bean mutant collection: morphological and molecular characterization. Hereditas 161, 37 (2024). https://doi.org/10.1186/s41065-024-00339-7
Pysyvä osoite
Tiivistelmä
Background: Plant mutagenesis creates novel alleles, thereby increasing genetic and phenotypic diversity. The availability of the faba bean (Vicia faba L.) reference genome and a growing set of additional genomic resources has increased the scientific and practical value of mutant collections. We aimed to genotype and morphologically phenotype a historical faba bean mutant collection developed and characterized by Jan Sjödin (1934–2023) over half a century ago in order to increase its value to researchers. The collection was genotyped using high-throughput single-primer enrichment technology (SPET) assays.
Results: We used 11,073 informative single nucleotide polymorphism (SNP) markers spanning the faba bean genome to genotype 52 mutant lines along with the background line, cv. Primus. A range of flower, seed, leaf, and stipule mutations were observed. The analysis of population structure revealed a shallow structure with no major subpopulations. Principal component and cluster analyses revealed, to a minor extent, that the mutants clustered by their phenotype.
Conclusions: The mutants’ phenotypic variation and shallow structure indicate that the Sjödin faba bean collection has the potential to play a significant role in faba bean breeding and in genetic and functional studies.
ISBN
OKM-julkaisutyyppi
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Julkaisusarja
Hereditas
Volyymi
161
Numero
Sivut
Sivut
8 p.
ISSN
0018-0661
1601-5223
1601-5223