Enrichment of bovine milk-derived extracellular vesicles using surface-functionalized cellulose nanofibers
Ukkola, Jonne; Pratiwi, Feby W.; Kankaanpää, Santeri; Abdorahimzadeh, Seyedamirhosein; KarzarJeddi, Mohammad; Singh, Prateek; Zhyvolozhnyi, Artem; Makieieva, Olha; Viitala, Sirja; Samoylenko, Anatoliy; Häggman, Hely; Vainio, Seppo J.; Elbuken, Caglar; Liimatainen, Henrikki (2022)
Ukkola, Jonne
Pratiwi, Feby W.
Kankaanpää, Santeri
Abdorahimzadeh, Seyedamirhosein
KarzarJeddi, Mohammad
Singh, Prateek
Zhyvolozhnyi, Artem
Makieieva, Olha
Viitala, Sirja
Samoylenko, Anatoliy
Häggman, Hely
Vainio, Seppo J.
Elbuken, Caglar
Liimatainen, Henrikki
Julkaisusarja
Carbohydrate Polymers
Volyymi
297
Sivut
9 p.
Elsevier BV
2022
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi-fe2022120268765
http://urn.fi/URN:NBN:fi-fe2022120268765
Tiivistelmä
The isolation of extracellular vesicles (EVs) from milk, a complex mixture of colloidal structures having a comparable size to EVs, is challenging. Although ultracentrifugation (UC) has been widely used for EV isolation, this has significant limitations, including a long processing time at high g-force conditions and large sample volume requirements. We introduced a new approach based on nature nanoentities cellulose nanofibers (CNFs) and short time and low g-force centrifugation to isolate EVs from various milk fractions. The flexible and entangled network of CNFs forms nanoporous, which entraps the EVs. Further, positively charged CNFs interact with anionic EVs through an electrostatic attraction, promoting their isolation with efficiency comparable with UC. The functionality and toxicity of isolated milk EVs were tested in Caco2 cells. Overall, the newly developed approach provides straightforward isolation and biocompatibility and preserves the natural properties of the isolated EVs, enabling further applications.
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