Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep
Pokharel, Kisun; Peippo, Jaana; Weldenegodguad, Melak; Honkatukia, Mervi; Li, Meng-Hua; Kantanen, Juha (2020)
Pokharel, Kisun
Peippo, Jaana
Weldenegodguad, Melak
Honkatukia, Mervi
Li, Meng-Hua
Kantanen, Juha
Julkaisusarja
Genes
Volyymi
11
Numero
4
Sivut
19 p.
MDPI AG
2020
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi-fe2020051229396
http://urn.fi/URN:NBN:fi-fe2020051229396
Tiivistelmä
The majority of pregnancy loss in ruminants occurs during the preimplantation stage,
which is thus the most critical period determining reproductive success. Here, we performed a
comparative transcriptome study by sequencing total mRNA from corpus luteum (CL) collected
during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287
genes were expressed in our data. Highly expressed autosomal genes in the CL were associated
with biological processes such as progesterone formation (STAR, CYP11A1, and HSD3B1) and
embryo implantation (e.g., TIMP1, TIMP2 and TCTP). Among the list of differentially expressed
genes, sialic acid-binding immunoglobulin (Ig)-like lectins (SIGLEC3, SIGLEC14, SIGLEC8),
ribosomal proteins (RPL17, RPL34, RPS3A, MRPS33) and chemokines (CCL5, CCL24, CXCL13,
CXCL9) were upregulated in Finnsheep, while four multidrug resistance-associated proteins
(MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized noncoding
RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the
flushing diet effect. The significantly upregulated TXNL1 gene indicated potential for embryonic
diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes
that are active in the CL during early pregnancy (including TXNL1, SIGLEC14, SIGLEC8, MRP4, and
CA5A).
which is thus the most critical period determining reproductive success. Here, we performed a
comparative transcriptome study by sequencing total mRNA from corpus luteum (CL) collected
during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287
genes were expressed in our data. Highly expressed autosomal genes in the CL were associated
with biological processes such as progesterone formation (STAR, CYP11A1, and HSD3B1) and
embryo implantation (e.g., TIMP1, TIMP2 and TCTP). Among the list of differentially expressed
genes, sialic acid-binding immunoglobulin (Ig)-like lectins (SIGLEC3, SIGLEC14, SIGLEC8),
ribosomal proteins (RPL17, RPL34, RPS3A, MRPS33) and chemokines (CCL5, CCL24, CXCL13,
CXCL9) were upregulated in Finnsheep, while four multidrug resistance-associated proteins
(MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized noncoding
RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the
flushing diet effect. The significantly upregulated TXNL1 gene indicated potential for embryonic
diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes
that are active in the CL during early pregnancy (including TXNL1, SIGLEC14, SIGLEC8, MRP4, and
CA5A).
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