Production of Norway spruce embryos in a temporary immersion system (TIS)
Välimäki, Sakari; Paavilainen, Laura; Tikkinen, Mikko; Salonen, Frida; Varis, Saila; Aronen, Tuija (2020)
Välimäki, Sakari
Paavilainen, Laura
Tikkinen, Mikko
Salonen, Frida
Varis, Saila
Aronen, Tuija
Julkaisusarja
In vitro cellular and developmental biology: plant
Volyymi
56
Numero
4
Sivut
430-439
Springer
2020
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi-fe2022101962501
http://urn.fi/URN:NBN:fi-fe2022101962501
Tiivistelmä
Somatic embryogenesis has already been used for Norway spruce (Picea abies (L.) Karst) embling production on a laboratory scale, but automation is needed to increase efficiency and reduce costs. One option to scale up production is mass production in bioreactors. In a series of experiments, a pro-embryogenic mass was propagated using Plantform temporary immersion system bioreactors, and the effect of different aeration cycles, support pad materials, and post-maturation treatments (rinsing and desiccation) on the embryo yield and embling survival after 4 to 6 mo in a greenhouse was tested. Three genotypes were used to test each treatment. The best aeration frequency was 20 min every 4 h, while a lower or higher frequency did not generally improve embryo production. Filter paper on plastic netting was the best support pad material in terms of usability and embryo production (varying from 177 ± 20 to 696 ± 109 per g pro-embryogenic mass). The separation of the embryos from the undeveloped cell mass by rinsing with sterile water resulted in reduced survival of the emblings. Desiccation treatment on nested plates with the embryos on the inner plate with or without filter paper improved their survival. Bioreactors were laborious to prepare, load, and clean. Improvements in embryo production can be achieved by optimizing the process, but bioreactors based on the requirements of somatic embryogenesis are needed to enable their use in the mass production of Norway spruce emblings.
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