Biochemical parameters of oats edaphic resistance

Abstract

As the aluminum resistance is multigene character, which defined by action of internal and external resistance mechanisms, it is necessary to know, what biochemical parameters are most suitable for a large-scale evaluation of aluminum- resistance level of oats varieties. On a set of oats varieties, differed on level of aluminum resistance, dynamics of development of such root indexes, as oxidative activity, activity of acidic phosphatase and acidic lipase, content and ratio of protein fractions during adaptive process was investigated. At utilized aluminum concentration (1 mM) dynamics of development of response was differed for different varieties. The most resistant variety Chizh has raised the level of root oxidative activity at 5th day of study and sustained it during all time of study. Less resistant varieties Argamak and Rodney E stabilized the index at 6th day of study, hybrid Tarak õ Krasnodarsky - only at 7th day, and the most sensitive variety I-2674 during all study displayed vibratory nature of a change of oxidative activity. Under stress condition there was significant modification of a content of protein fractions for all studied varieties, in most cases dynamics of the modification had vibratory nature. On 5th and 8th day of aluminum action, the relative activity of acidic phosphatase of 'Argamak' was significantly higher, but on 6th and 7th day - lower, than for 'Chizh'. The relative activity of an acidic lipase also alternated on days: on 5th and 7th day, it was higher for variety 'Chizh', on 6th and 8th day - for variety 'Argamak'. The variety 'Chizh' exceeded variety 'Argamak' in all studied indexes only at 7th day of study, at remaining days the variety 'Argamak' exceeded variety 'Chizh' in some indexes. Thus, different biochemical parameters utilized at different phases of adaptive process, can essentially influence on evaluation of a level of plant aluminum resistance. The most informative may be studying the dynamics of development of exact parameter at 5-8 days of study: the earlier biochemical index will return to control level the most resistant variety will be.